Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIRRIEGLLHLASEGRYAILVGRFNSFVVEHLLEGAIDTLKRHGVNEDNITVIHAPGAWELPIVAKKLATSNQFDAIIALGAVIRGSTPHFDFVAGECAKGLGVVALESSLPVINGVLTTDSIEQAIERSG--TKAGNKGSEAALTAIEMVNLLKAI 2C92 Chain:E ((15-139)) ------------ASGVRLAIVASSWHGKICDALLDGARKVAA--GCGLDDPTVVRVLGAIEIPVVAQELARNH--DAVVALGVVIRGQTPHFDYVCDAVTQGLTRVSLDSSTPIANGVLTTNTEEQALDRAGLPTSAEDKG-----------------

General information: TITO was launched using: RESULT: Template: 2C92.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 582 -99220 -170.48 -806.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain E : 0.77 3D Compatibility (PKB) : -170.48 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.654

(partial model without unconserved sides chains): PDB file : Tito_2C92.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2C92-query.scw PDB file : Tito_Scwrl_2C92.pdb: