Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKYALGPVIGEGQFGSVRMATVKATGQTVAVKLLHVPRLTEGIPHPVARELLIASRVASPFLVKTIEIAPYGSHMAVVMERCMEDLACVLRKCSPAHPLPLLLTQSYMRMLLTALHALHSSGILHRDVKPSNCFVAVDGCLKLGDFGLSRPLCSDMSHEVVSRWYRAPELLFGQRRYGGEVDLWAAGCVFAELLRGHGRPFFTGDGDISQIAKIFDVFGTPTGGTCSFYRQLPDWEKVSFEEKKGTGLRVLLPFVPPEALDLLTKMLALDPASRCSAAEALSHPFFALSDILLRV 1GIH Chain:A ((1-286)) MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIR--------PSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFM-DASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLA-----------VTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT--RRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDY-KPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFF---------

General information: TITO was launched using: RESULT: Template: 1GIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1258 -179573 -142.74 -680.20 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -142.74 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_1GIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GIH-query.scw PDB file : Tito_Scwrl_1GIH.pdb: