Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---PVNLQEVRSNDQQL-HPYRCKFEV------LHIWYTE--PFLSSFIDPGQRAEITIEARARKTDFAIDIDGE-CKGTKVKGAGRI---PENWFIRLSREGSKEVQFINDKPKNNRVSGGS- 1UIT Chain:A ((1-117)) GSSGSSGGERRKDRPYVEEPRHVKVQKGSEPLGISIVSGEKGGIYVSKVTVGSIAH---QAGLEYGDQLLEFNGINLRSATEQQARLIIGQQCD-TITILAQYNPHVH---QLSSHSRSGPSSG

General information: TITO was launched using: RESULT: Template: 1UIT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 386 20288 52.56 202.88 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 52.56 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_1UIT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UIT-query.scw PDB file : Tito_Scwrl_1UIT.pdb: