Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MKDMMGN-GFIANAEAFTLATQIYVRMRRVTGRV---IDAMYVVQNKDYAKY-VIAL-ALEAEDDELKRCVERLMVLTDSIPEQF-QKE-M-TTVSIQTSEESEITAEDI-------YRAPVPH-HYIGALR 3FH3 Chain:A ((12-157)) KHAGTMTLEAYMRFSAKLSEAKDEMG-TKEYEVFTKELKKLTNAKLAYGDSNGNID--YDALSSEKREEMKKVSMGLQPYFDKLNGHKSSKEVLTQEEFDRYMEALMTHEIVRVKTKSTGAIKVEEIPEAYKERFIKAEQFMEYVDEKV

General information: TITO was launched using: RESULT: Template: 3FH3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 270 24651 91.30 220.10 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 91.30 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_3FH3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FH3-query.scw PDB file : Tito_Scwrl_3FH3.pdb: