Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSQEPNQSLFNQASTSIQACIECMFACKHCASACLQEEHVHMMRKCIKHCLTCVETCQLCTSLELRHSELAEQAMQLCANACQLCAAECSKHEHEHCQVCAKACLACAQACQAYRA 5FIG Chain:E ((4-108)) -------------YSEACIEACIDCMKACNHCFTKCLE-----HLSGCIRLDRECADICALAVKAMQTDSPFMKEICALCADICEACGTECGKHDHDHCQACAKACFTCAEQCRSMAA

General information: TITO was launched using: RESULT: Template: 5FIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 475 -79683 -167.75 -796.83 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain E : 0.79 3D Compatibility (PKB) : -167.75 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.638

(partial model without unconserved sides chains): PDB file : Tito_5FIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FIG-query.scw PDB file : Tito_Scwrl_5FIG.pdb: