Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSTAKHNYAPDILGAGYEQLTLNFPDDYEGKVVATLVRKKATQPTQKAVLYIHGFLDYFFQTE-MAEQFNAHGYDFYALDLRKYGRSKLPHQIFYNVLDLIEYDAEITQALEIIGQEKHTQVLLAGHSTGGLTATLYAAHHPDHPLIKALWA-NSPFYDFNLSLVEKKFGIPMLSRVGKYLPKVKFPSQLNKWYTTSLHKQLKGEWDFNLDWKPTSAPTVQLSFVHAIHTAQKEIHRGVKLNIPALIMHSHQTKNPRKWGPDATQSDVILDVKDIAKFGKKMKGDVSVVSIHNGLHDLVLSAQPVREQVYQQLFQWLDQKVT 1ZD2 Chain:P ((260-366)) -------------------------------------------------AVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVL--CKEMVTFLDKLG---LSQAVFIGHDWGGMLVWYMALFYPER--VRAVASLNTPFIPAN-----------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZD2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 472 -41520 -87.97 -395.42 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain P : 0.60 3D Compatibility (PKB) : -87.97 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_1ZD2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZD2-query.scw PDB file : Tito_Scwrl_1ZD2.pdb: