Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSPSNPVLSHHISSQFNEDLQDVNTKFMTMGGLVEQQVANAIHSLLDT-------DANLAIDVQFKDNAVNQYERDIDEGLTLILARRHPAAIDLRMVIAMSKANTDLERIGDEAAKIARIAQNLCEEGGSPRGYMETRHIGNQVRVMI---HDALDAFAR--------LD----ADQALRVLLADVDIDREYQSATRTLMTYMIEDPRHIARVINVMWVLRSLERIGDHARNIAEQVIYMAKGFDARHTKIEEIEAKVHEK 2OLT Chain:C ((14-224)) ---------------------SPIKPLQEHMDKVYDCASLLVPFFEATITGNWDDAVQIRKQISLAEKQGDSLKREIRLTL--GL--FMPV--ERT---DLLELLTQQDKIANKAKDISGRVIGRQLL-IPQALQVPFIAYLQRCIDAVGLAQQVINELDDLLEAGFRGREVDFVAKMINELDIIEEDTDDLQIQLRRQLFALES--ELNPVDVMFLYKTIEWVGGLADLAERVGSRLELML--------------------

General information: TITO was launched using: RESULT: Template: 2OLT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 622 -32590 -52.40 -174.28 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.61 3D Compatibility (PKB) : -52.40 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_2OLT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLT-query.scw PDB file : Tito_Scwrl_2OLT.pdb: