Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSNQNDKLKSLKTSSMDRRLSIAKASLL-AGTRWATASASSLFSSEEEKEKKRKKAMSEQANYLVSEIGK-LKGSIVKIGQMMALYGEHFL---PEEITQALNTLNNQTVALNWPAIKPHLQEQLGSKLNDLTI-DHEPIGTA-SLAQVHCATRKSDGLELVLKIQY-PG---VANAIDSDMSLFKNMLKLTRMVPQTREFDQWFDEVREMMHREVNYHVEAA----------TTRRFAERLKDDERYIVPTIVDEYCTDQVLCMTFERGVPINSPVMLSLPQERRNQLGEASLEIAVREIFEWGEMQTDPNFGNYLVRL---GNGQD-IQDKIVLLDFGAIRQFDEHLLSVARNLIQAGYQHDKHAMVQAMTGYEFFDAMPESIKPGMADVFLLATEAFSTPANNPDMPAGLMDEHERYDWKKSQLHSRVMQQASKSMASR--YFSVPPKEFMFISRKFIGAYTFMTVIDAKTNVRRMIRNFA 2VOS Chain:A ((22-487)) DEIASLLQVEHLLDQRWRIDPSLTRISALMDLLGSPQRSYPSIHIA---------------GTNGKTSVARMVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQYVATYREIEPLV-ALIDQQSQASPAMSKFEVLTAMAFAAFAD-----APVDVAVVEVGMGGRWDATNVINAPVAVITP-ISIDHVDYLGADIAGIAGEKAGIITRAPSPDTVAVIGRQVPKVMEVLLAESVRADASVAREDSEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFF-GAQLDGDAVRAGFAAVTSPGRLERMRSAPTVFID-AA---HNPAGASALAQTLAHEFDFRFLVGVLSVLGDKDVDGILAALEP-VFDSVVVTHNGSPRALDVEALALAAGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAGRTGIVIT----GSVVTAGAARTLFGRDPQ-----

General information: TITO was launched using: RESULT: Template: 2VOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2418 24599 10.17 58.57 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 10.17 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_2VOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VOS-query.scw PDB file : Tito_Scwrl_2VOS.pdb: