Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTQNLMCPVCRQRLELVSKTWRCEQGHSYDIAKQGYVNLHVVQHKHSKNPGDTPESVDARRAFLQGGYYQPLQQA--------VVHLLKDLQAKMVLDIGCGEGYYTSAM-QQVVEQCIGVDIAKNAVQRA----AKLNDKVTWVVGTGATLPVIDQSMDVCTS--LFSPIPQTEILR--------VVKDDGYLIVVTSATDHLYAMREALFEQVNPHIPQKFVEQLQDLFELKEQQVIDAPLVLDQQALKNLIAMTPYAYKASPERRMQLEQKAHLQVTASFQIYLFQKRNKKAI 5JE3 Chain:A ((25-153)) ----------------------------------------------------------------------------QRAIEVRTIFHMIGDVSGKSVLDLACGFGFFGREIYRRGAAKVVGVDISEKMIELAREESRKYGDPLEFHVRDVANMEPLGQ-FDLVNAAWLFNYADSVENLRKMFKVVRASLKPDGKLVAYTVDPD----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5JE3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 437 -4255 -9.74 -41.31 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -9.74 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_5JE3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JE3-query.scw PDB file : Tito_Scwrl_5JE3.pdb: