Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSIVYAFVGGLLLGIATVGYLYINGRIAGISGLLAQFISPSRDIFKSSAFWFIAGLVITPFI---YGYFYQPEIEIKANSFVLILAGLLVG--FGTRLGSGCTS-GHGICGMSRLSK----RSIIASAIFMFAGMLTVYIIRHVLG 1NIG Chain:A ((1-148)) MDIKRYCPVTDSELP-ADHVYFKFRSEIEAAEAYLGLAISEGIKVRETREILDIIDTVYNSLSDSKLNDFQEKRLNFTEEDWYDIKEKANNGNRWSLYMFLARSAVDSAVYWSYRMKETEEFKEIVKEEMISKLLKAGYVILRESLG

General information: TITO was launched using: RESULT: Template: 1NIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 480 -54926 -114.43 -403.87 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -114.43 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.242

(partial model without unconserved sides chains): PDB file : Tito_1NIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NIG-query.scw PDB file : Tito_Scwrl_1NIG.pdb: