Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLASLIGGIFGTKNERELKRMRKIVEQINALEPTISALSDADLSAKTPEFKQRYNNGESLDKLLPEAFAVCREAAKRVMGMRHYDVQLIGGITLHEGKIAEMRTGEGKTLMGTLACYLNALSGEGVHVITVNDYLAQRDAELNRPLFEFLGLSIGTIYSMQEPAEKAAAYLADITYGTNNEFGFDYLRDNMVFSLAEKKQRGLHYAIIDEVDSILIDEARTPLIISGQSEDSSHLYTAINTIPPKLRPQKEEKVADGGHFWIDEKQRSVEMTEIGYETVEQELIQMGLLAEGESLYSATNLNLVHHVSAAIRAHVLFQRDVHYIIHDGEVVIVDEHTGRTMPGRRWSEGLHQAVEAKEGLAIQPENQTLATTTFQNYFRPYKKLSGMTGTADTEAAEMKEIYGLDVVIIPTHRPMIRNDQNDLIYLNRNGKYNAIIQEIMNIRQQGVAPILIGTATIEASEILSSKLKQAGIHHEVLNAKQHEREADIIAQAGSPNAVTIATNMAGRGTDIILGGNWKAKLAKLENPTPEDEARLKAQWEQDHEDVLQAGGLHIIGSERHESRRIDNQLRGRAGRQGDPGVSRFYLSLEDDLMRIFAGDRVVAMMRAMGLKEDEAIEHKMVSRSIENAQRKVEARNFDIRKNLLKYDDVNNEQRKIIYSQRDEILAENTLQEYVEEMHREVMQAMIANFIPPESIHDQWDVEGLENALRIDLGIELPVQEWLEQDRRLDEEGLVERISDEVIARYRQRRAQMGDESAAMLERHFVLNSLDRHWKDHLAAMDYLRQGIHLR--------------GY----------------------------AQKNPEQEYKKESFNLFVNMLGVIKTDVVTDLVTDLSRVHIPTPEELAEMEAQQQQQAEAMKLSFEHDDVDGLTGEVTASQEALNESATEQQTFPVPESRNAPCPCGSGLKYKQCHGKI 5EUL Chain:A ((14-818)) ---------------RTLNRYEKIANDIDAIRGDYENLSDDALKHKTIEFKERLEKGATTDDLLVEAFAVVREASRRVTGMFPFKVQLMGGVALHDGNIAEMKTGEGKTLTSTLPVYLNALTGKGVHVVTVNEYLASRDAEQMGKIFEFLGLTVGLNLNSMSKDEKREAYAADITYSTNNELGFDYLRDNMVLYKEQMVQRPLHFAVIDEVDSILIDEARTPLIISGQAAKSTKLYVQANAFVRTLK------------YTYDIKTKAVQLTEEGMTKAEKAF---GI----DNLFDVKHVALNHHINQALKAHVAMQKDVDYVVEDGQVVIVDSFTGRLMKGRRYSEGLHQAIEAKEGLEIQNESMTLATITFQNYFRMYEKLAGMTGTAKTEEEEFRNIYNMQVVTIPTNRPVVRDDRPDLIYRTMEGKFKAVAEDVAQRYMTG-QPVLVGTVAVETSELISKLLKNKGIPHQVLNAKNHEREAQIIEEAGQKGAVTIATNMAG--TDIKLG-----------------------------EGVKELGGLAVVGTERHESRRIDNQLRGRSGRQGDPGITQFYLSMEDELMRRFGAERTMAMLDRFGMDDSTPIQSKMVSRAVESSQKRVEGNNFDSRKQLLQYDDVLRQQREVIYKQRFEVID-----EIVENMIKSSLERAIAAY-------------------------------------------MLELIMDRIITKYNE------KEQMREFEKVIVLRAVDSKWMDHIDAMDQLRQGIHLRGSKKTAIAIAVALAGFATVASYAQYEDGCSGELERQHTFAGGPGA---PLREYQMEGFAMFEHMIESIEDEV-------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EUL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3485 -209744 -60.18 -300.92 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -60.18 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_5EUL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EUL-query.scw PDB file : Tito_Scwrl_5EUL.pdb: