Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQYQPPHINRKLNVQAWWLTALLISINVGLFIWQIISGVDISDPAIKDALHWGADFTPLTFSGQPERLFTSMFFHFGIIHLLLNMWALYIFGNVAEALFGRLYFIGLYLLAGLFGSLLSSYINIQNGHELLQHFDQSLLPHVSAGASGAVMGLGAALTVLSLFPPLPHQAYILDKKALLMIMAINLI---FGFVATGINNAAHVGGMIMGALLALIWYLSYRTPFKSLLKFLGLVGGLFMTVGFYVYCNQLNSPLLPLWHEVLIQNPEILP 2XOW Chain:A ((36-176)) --------------------------------------------------------FDP-TLKFEFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGS----GKLIVITLISALLSGYVQ--------QKFSGPWF----GGLSGVVYALMGYVWLRGERDP---QSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFV-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2XOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 581 -101458 -174.63 -735.20 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -174.63 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_2XOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XOW-query.scw PDB file : Tito_Scwrl_2XOW.pdb: