Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFYKHTSLLAERITAHGALPNTSYWRGFFFSGVCMTEFQKITHEIRQLQVELNHLGSCNTKGLTTEQIAHLDERFFLAIAKQNKLLARLNNKPEGFL 3FF5 Chain:B ((39-50)) -----------------------------------------------------------KKGLTDEEIDLA--------------------------

General information: TITO was launched using: RESULT: Template: 3FF5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 10 -871 -87.10 -72.58 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -87.10 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 1.480

(partial model without unconserved sides chains): PDB file : Tito_3FF5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FF5-query.scw PDB file : Tito_Scwrl_3FF5.pdb: