Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPYQPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIPAIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPYIANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLEQLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEYLSQKDLTRILDILNIQFDEE 1R71 Chain:D ((22-91)) ------------------------------------------------------------------------------------------------------------NEADQ----VIENLQRNELTPREIADFIGRELAKGK-KKGDIAKEIGKSPAFITQHVTLLDLPEKIADAFNTGRV-----------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1R71.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 190 -18507 -97.40 -264.38 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain D : 0.61 3D Compatibility (PKB) : -97.40 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.688

(partial model without unconserved sides chains): PDB file : Tito_1R71.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R71-query.scw PDB file : Tito_Scwrl_1R71.pdb: