Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHTTQLYQHVPETRWPIVYSPRYNITFMGLEKLHPFDAGKWGKVINFLKEEKLLSDSM-LVEAREASEEDLLVVHTRRYLNELKWSFAVATITEIPPVI---FLPNFLVQRKVLRPLRTQTGGTIMAGKLAVERGW--AINVGGGFHHCSSDRGGGFCAYADITLAIKFLFERVEGISRATIIDLDAHQGNGHERDFMDDKRVYIMDV--YNRHIYPGDRFAKQAIRRK-------VELEWGTEDDEYLDKVERNIKKSLQEHLPDVVVYNAGTDILEGDRLGGLSISPAGIVKRDELVFRMVRGRRVPILMVTSGGYQ-KRTARIIADSILNLFGLGLIGPESPSVSAQNSDTPLLPPAVP 1T64 Chain:A ((19-314)) ------------------IYSPEYVSMCDSLAKI-----PKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQ-KVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFE---RILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGK----CLKYILQWQLATLILGGGGYNLANTARC------------------------------------

General information: TITO was launched using: RESULT: Template: 1T64.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1568 -12196 -7.78 -43.56 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -7.78 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_1T64.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T64-query.scw PDB file : Tito_Scwrl_1T64.pdb: