Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------TVASRRDDCWVSLFQYKANGEREAIYVDYSPPNSLVIFVHDGWRLNVQLDQHCRSLFPNDIRKQLVEGFGVHSYSKMNEVAGQKPLARSQ------------------------------------------------ 5LDD Chain:B ((13-248)) IIPAQLGFLAIYNPALGTTDETLEDQIVYYATASTLSPVSKEERHERLRQIGLAQGMVEFAKSFSDGEPVD-TIDTEKARVILVEVEEGWWILASIDL---TRLPYEYSSREVK---------------PPSLLRADLLRAYDLFLLHHGSSLSSLLASQGRAQLVASLTRFWDHFLATWNVLLH

General information: TITO was launched using: RESULT: Template: 5LDD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 151 7517 49.78 105.87 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : 49.78 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.080

(partial model without unconserved sides chains): PDB file : Tito_5LDD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LDD-query.scw PDB file : Tito_Scwrl_5LDD.pdb: