Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PSDGPEAPEELCFTTIIRRRDGFKYAEKGPIGQKIAFLAGLNCTVQKGC---KLAC-TPAHYGLKYKVSCRPA------- 2KSW Chain:A ((1-66)) VPVGSDCEPKLC--TMDLVPHCFLNPEKG------------IVVVHGGCALSKYKCQNPNHEKLGYTHECEEAIKNAPRP

General information: TITO was launched using: RESULT: Template: 2KSW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 212 655 3.09 11.91 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 3.09 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_2KSW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KSW-query.scw PDB file : Tito_Scwrl_2KSW.pdb: