Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------PWNNYCQLTLMRGRQIQAQSYLVR-RSFPLTTVVIRLEPGKSRSF--KLNWNCRLKIPRGVVPEGHWLYADMKYMTPSMAADLNNLLDGAPWPLEWPLDRESLRLESQRNSRVFQS------------------------------------ 2X1W Chain:L ((4-207)) SPFIASHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKSYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQ-------------------HKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHE

General information: TITO was launched using: RESULT: Template: 2X1W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 446 -11458 -25.69 -121.89 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain L : 0.57 3D Compatibility (PKB) : -25.69 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.239

(partial model without unconserved sides chains): PDB file : Tito_2X1W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X1W-query.scw PDB file : Tito_Scwrl_2X1W.pdb: