Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELAETSIVKKNHQIPCIINQKIAQKLIEKTSMTDIDHQLSISTSTVIRKINNFHFEHDFSRLPEIMS 2M8E Chain:A ((7-50)) ------------KEISQDLRKKIVDLHKSGSSLGAISKRLKVPRSSVQTIVRKYKH------------

General information: TITO was launched using: RESULT: Template: 2M8E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 76 -14122 -185.81 -320.94 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -185.81 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_2M8E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M8E-query.scw PDB file : Tito_Scwrl_2M8E.pdb: