Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MRNIGQAGKILADSG----------------------------------------YQGLMKIYPQAQTPRKSSKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF 4EPC Chain:A ((696-847)) NNQLTVTNNSGVAQINANSGLYTTVYDTKGKTTNQIQRTLSVTAATLGDFYLVGDYNTGTNYGWVQDEVIYNTASPVKINQTYNVPGVLHTVPWGTYNQVAGTV-SGKGDQTFKATQQQIDATYLYGTVNGKSGWISYYLTA

General information: TITO was launched using: RESULT: Template: 4EPC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 351 30446 86.74 317.14 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 86.74 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.069

(partial model without unconserved sides chains): PDB file : Tito_4EPC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EPC-query.scw PDB file : Tito_Scwrl_4EPC.pdb: