Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMERNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGRRLQKEIFGE 1OWF Chain:B ((15-42)) -------------------------------SHIPAKTVEDAVKEMLEHMASTLAQGER---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1OWF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 10 827 82.70 29.54 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.49 3D Compatibility (PKB) : 82.70 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_1OWF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OWF-query.scw PDB file : Tito_Scwrl_1OWF.pdb: