Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKLEVKDNKKLVLKSVICKKLHDTKV--------EDVDQEINRFHQHLQLLKAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVRFEDSP-EYLQYAYSKLDL-YVYEN-DIQTD-GIVYTVYVNSSPEKMVVDIFIPIVSL 3GK6 Chain:A ((25-170)) ---------SEINGFEVTGFVVR-TTNADEMNPMTAKIGNLWEKFYLNA-APKLTDKSKVYGLYTNYESDF--TGAFDVIACSDTLSP-QLLSESVKTKVSS-GKYVTFSATGEMPQVVIDLWNEVWNYFA---CPHKRAYTTDFEYYKSAN----TVEISIAVR--

General information: TITO was launched using: RESULT: Template: 3GK6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 519 -14557 -28.05 -111.12 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -28.05 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_3GK6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GK6-query.scw PDB file : Tito_Scwrl_3GK6.pdb: