Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MKDDQKYLLA---------GLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLIYFI------ATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPYGLL-KKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLF-----VQSYLGSNTAWIGNR--RLII----------DDEFEK----ILLSKSYVKE--ISSARYSEYLTCEYKNLTPIILIAIFENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFN---------YFVSLNYLLYK----------VGFLGIIYDNEALRDLSKQYLNK-NISELQDGSFEGGIQDAVASKQIVVINEFI---ACLNSRCVPSQYD----RFFYKDRPYIFSRKSPIKG----- 5IX1 Chain:A ((3-450)) GIRLSALCPKFLHTNSTSHTWPFSAVAELIDNAYDPDVNAKQIWIDKTVISDHICLTFTDNGNGMTADKLHKMLSFGFSDKVTMNGHVPVGLYGNGFKSGSMRL------GKDAMVFTKNGE---TMSVGFLSQTYLEVIKAEH----VVVPIVTFNKHRQMINLTESKASLAAILEHSLFSTEQKLLAELNAIMGKKGTRIIIWNLRSYKNATEFDFEKDKYDIRIPEDYKKQEIAPESDYSLRAYCSILYLKPRMQIIIRGQKVKTQLVSKSLAYIERDVYRPKFLTRTVRITFGFNCRNKDHYGIMMYHKNRLIKAYEKVGCQNMGVGVVGIIECNFLKPTHNKQDFDYTNEYRLTILALGEKLNDYWNEMKKRPDQTWVQCDACLKWRKLPDGIDQLPEKWYCSNNPDPQFRNCEVPEEPEDE

General information: TITO was launched using: RESULT: Template: 5IX1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1775 -84424 -47.56 -249.04 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -47.56 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.119

(partial model without unconserved sides chains): PDB file : Tito_5IX1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IX1-query.scw PDB file : Tito_Scwrl_5IX1.pdb: