Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTVLEAYQIVSSIHR--RNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQALRNR-TPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLAILCNYANNLANTPINPELQPYA 2OYO Chain:B ((13-194)) ISSLPVPDATQVPEGVRKLWAKAEANIG-FVPNVFRAQAVNGEQFLAWWNYFNLLLNKEGYLTNAERELVAVVVSGVNRCLYCAVSHGAALR-EFLGDPQKADAVAVNWRHADLTEREQALAAYAEKLTRHPAEVTAADLEPLRAVGLDDHQIMELVQVIGMFNLTNRVSSALGFVPNPEYYRQ-

General information: TITO was launched using: RESULT: Template: 2OYO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 774 -42288 -54.64 -236.24 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -54.64 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_2OYO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OYO-query.scw PDB file : Tito_Scwrl_2OYO.pdb: