Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNGAVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNEKLKLYLASDLTKVE-NPRPQDEDETLEVLEVSLEEAKELIQSGHI-CDAKTIMAVQYWELQKK 3X0J Chain:A ((11-164)) ---------RTYLYRGRILNLALE----------GRYEIVEHKPAVAVIALR-EGRMLFVRQMRPAVGLAPLEIPAGLIEPGED--PLEAARRELAEETGLSGDLTYLFSYFVSPGFTDEKTHVFLAENLKEVEAH-----EDEAIEVVWMRPEEALERHQRGEVEFSATGLVGVLYYH----

General information: TITO was launched using: RESULT: Template: 3X0J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 686 -55796 -81.34 -371.97 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -81.34 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_3X0J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3X0J-query.scw PDB file : Tito_Scwrl_3X0J.pdb: