Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNCTPKVRQKKSNFWGVFIMKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDHYGIEFVKKGKNRYYSPDLKQEMIHKV 2ELH Chain:A ((19-67)) -----------------PLRSLTPRDKIHAIQRIHDGESKASVARDIGVPESTLRGWCKNEDKLRF-----------------------

General information: TITO was launched using: RESULT: Template: 2ELH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 114 -12883 -113.01 -262.92 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -113.01 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.682

(partial model without unconserved sides chains): PDB file : Tito_2ELH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ELH-query.scw PDB file : Tito_Scwrl_2ELH.pdb: