Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKELHINTILAQAGIKSDEATGALVTPLHFSTTY--QHPEFGRS--TGFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAF-SVFPVGSKVLAVRDLYGGSFRWFNQVEQEGRFHFTY--ANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAKLAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNL-NTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTG----------------RGGMISFKVADETRIP-HILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQALEG 5K30 Chain:A ((10-393)) -------NTQIVHAGQQPDPSTGALSTPIFQTSTFVFDSAEQGAARF------RLGNPTTDALEKKLAVLERGEAGLATASGISAITTTLLTLCQQGDHIVSASAIYG-THAFLSHSMPKFGINVSFVDAAKPEEIRAAMRPETKVVYIETPANPTLSLVDIETVAGIAHQQGALLVVDNTFMSPYCQQPLQLGADIVVHSVTKYINGHGDVIGGIIVGKQEFIDQARFVGLKDITGG-MSPFNAWLTLRGVKTLGIRMERHCENALKIARFLEGHPSITRVYYPGLSSHPQYELGQRQMSLPGGIISFEIAGGLEAGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPVAPEERLKAGITDGLIRLSVGLEDPEDIINDLEHAI--

General information: TITO was launched using: RESULT: Template: 5K30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1878 -18967 -10.10 -54.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -10.10 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_5K30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K30-query.scw PDB file : Tito_Scwrl_5K30.pdb: