Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------MKIPLLTFA-------------RHKFVYVLLTLLFLALVYRDVL----MTYFFF-DIHAPDLAKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRL-------SIGREVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFS---------PLGWNSLFSDG-----------------------SGLQRLLDGEIKSY------LFFTCVLLIGIFINAIYFLQIV--------DYVGNVT----RSAITYLMFLWL-----GSMLLYSALPYYMV-----PMTSLMQASYGDVSLMKLFT----PYILYIVPYMVLEKYEDNV----------------- 4OC8 Chain:A ((2-388)) TFFTGETLGQVDLIVDAVYAGYKTERGGMADPLVPLVGVSRQGGFRYRGTRERPTLLVLTSNLAEPEWPDQLDETTGTFIYYGDNRHPGRLLHDTPRFGNQLLRQIFDWAHLG------QRHLVPPILVFTT----EATGRTFRFRGLAVPGSPALAATEDLVALWKTTEGQRFQNYKAVFTILDEAVIPRAWVHAVGRGETSGLAPVAWNAWLSAGGIRPLMAPRSLLVRSKAEQLPATPEDQALIEVIRQRYKENPFGFEACAGALTRLLLPDVARLDLTRPWRDGGRDGIGRLRIGQSPAAIEVDFALEAKCYGANNAVGVKEVSRLISRIKHREFGVLVTTSYVDRQAYQEVTDDGHPVILTTAQDIVGLLRSAGVRTPTQVDAWLDGITASV

General information: TITO was launched using: RESULT: Template: 4OC8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1143 -120690 -105.59 -477.03 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -105.59 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_4OC8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OC8-query.scw PDB file : Tito_Scwrl_4OC8.pdb: