Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MRFEVLSTSLIVTKIVFFVR- 5U3N Chain:A ((4-26)) ELDKWASLWNWFDITNWLWYIRK

General information: TITO was launched using: RESULT: Template: 5U3N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 6 32 5.33 1.60 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 5.33 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.76 QMean score : -0.262

(partial model without unconserved sides chains): PDB file : Tito_5U3N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U3N-query.scw PDB file : Tito_Scwrl_5U3N.pdb: