Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLGNVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEIGDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAEPVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQTQQKTALEDALRTL 4EA8 Chain:A ((99-215)) -------------------------------------------------------------------------------------VNA-IHPSAVVSPSVRLGEGVAVMAGVAINADSWIGDLAIINTGAVVDHDCRLGAACHLGPASALAG--------GVSVGERAFLGVGARVIPGVTIGADTIVGAGGVVVRDLPDSVLAIGVPAKI---------------------

General information: TITO was launched using: RESULT: Template: 4EA8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 714 -132477 -185.54 -1132.28 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -185.54 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_4EA8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EA8-query.scw PDB file : Tito_Scwrl_4EA8.pdb: