Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRTRMSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSGIDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVLACLREPLPASHYMIAVAGKD-VRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAGAQNLLNAFNIVEEVEYCAKIYCLAKNFGEPV-VLPDEEMELMAEKFKTYGQRK 1E49 Chain:P ((1-206)) MERNKLA---RQIIDTCLEMTRLGLNQGTAGLVSV--RYQDGMLITPTGIPYEKLTESHIVFIDGNGKHEEG-KLPASEWRFHMAAYQSRPDANAVVHNHAVHCTAVSILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIACEVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKF-------

General information: TITO was launched using: RESULT: Template: 1E49.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1108 -86312 -77.90 -423.10 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain P : 0.83 3D Compatibility (PKB) : -77.90 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_1E49.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E49-query.scw PDB file : Tito_Scwrl_1E49.pdb: