Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MWIAISALNLAFAVMLGAFGAHGLKAHASPEQLAWWQTATDYFFYHALGLLALGILSKVLPHFPIKLSFLLIQIGILFFCGSLYIMALGLPRILGAITPIGGALMIAGWLILAWNAFKYAK 5J9T Chain:D ((1-119)) MDPSLVLEQTIQDVSNLPSEFRYLLEEIGSNDLKLIEEKKKYEQKESQIHKFIRQQ----------GSIPKHPQEDGLDKEIKESLLKCQSLQREKCVLANTALFLIARHLNKLEKNIALLEEDGVLAP-

General information: TITO was launched using: RESULT: Template: 5J9T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 195 -1352 -6.93 -12.29 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain D : 0.57 3D Compatibility (PKB) : -6.93 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_5J9T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J9T-query.scw PDB file : Tito_Scwrl_5J9T.pdb: