Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGKLFGDRSYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLRKR----GIIEAPFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA 3C16 Chain:C ((128-221)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------VDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDV-IKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHM----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3C16.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 144 -17887 -124.22 -198.74 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain C : 0.54 3D Compatibility (PKB) : -124.22 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.131

(partial model without unconserved sides chains): PDB file : Tito_3C16.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C16-query.scw PDB file : Tito_Scwrl_3C16.pdb: