Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQGFIKAFANLVGWIFALIMGAKYAVLLAPSMSGLSQDPVVQKIAAFAFIALLIIVLTWIVTAFLNGLLKSLKLGPLNRLAGGAFGSLKGLLVVLITMQGVGPWVESSPHWKQSKFIQFLLPYAPLATELSKDAASEAFHQITSGGGVTRTSPKPMDESEETELRPDHSTKDPFY 2LV6 Chain:B ((1-26)) ---------------------------------------------------------------------------------------------------------------------------KRRWKKNFIAVSAANRFKKISSSGAL---------------------------

General information: TITO was launched using: RESULT: Template: 2LV6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 10 391 39.05 15.02 target 2D structure prediction score : 0.88 Monomeric hydrophicity matching model chain B : 0.46 3D Compatibility (PKB) : 39.05 2D Compatibility (Sec. Struct. Predict.) : 0.88 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_2LV6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LV6-query.scw PDB file : Tito_Scwrl_2LV6.pdb: