Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIEMFCELLS-LLGLKVVPKDYQSIDKERVAALL---VMSKSWMNRIETVD-----DLFHDEISCQKEKLGY 4BPT Chain:B ((63-123)) --QLPEVSERLKAKTGWQVAPVAALISAREFFELLAEKYFPAATFIRSEEELDYVQEPDIFHE-----------

General information: TITO was launched using: RESULT: Template: 4BPT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 106 -12419 -117.16 -238.83 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -117.16 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_4BPT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BPT-query.scw PDB file : Tito_Scwrl_4BPT.pdb: