Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MHEITLHEVRQLIAS-----LRTVYAAQFNKQFPTSGESAIPLSVVEQIALKTLVGVQQNQFNNALARLLTAGGRFMPSFAEFRTWCIGESWMSPEEAWSRACKFTTDRTVVITQITKYALDEVMYLIEAGQMRAAQDNFFGTYNVMVAKAQLKGRQQEFYTPPLQLEHKEPEHTPVSNDEV--QKQLKSLMERLKINGRKPAPVQKLQAKEKEPELAKELGPDPFDNPHEYAEMCRREGMPIPRNILQLIEGANV 1EFP Chain:B ((1-246)) MKVLVPVKRLIDYNVKARVKSDGSGVDLANVKMSMNPFDEIAVEEAIRLKEKGQAEEIIAVSIGVKQAAETLRTALAMGAD---RAILVVAADDVQQDIEPLAVAKILAAVARAEGTELIIAGKQAIDNDMN--ATGQMLAAILGWAQATFASKVEIEGAKAKVTREVDGGLQTIAVSLPAVVTADLRLNEPRYASLPNIMKA---KKKPLDEKTAADYGVDVAPRLEVVSVREPEGRKAGIKVG--SVDELVGKL------

General information: TITO was launched using: RESULT: Template: 1EFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1274 42878 33.66 184.03 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : 33.66 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.121

(partial model without unconserved sides chains): PDB file : Tito_1EFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EFP-query.scw PDB file : Tito_Scwrl_1EFP.pdb: