Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQYMTHRCLIAPPEMADDFFANTVIYLARHDEEGAQGIIINRPAGIQIKELLNDLDIDADNVNPHEVLQGGPLRPEAGFVLHTGQPTWHSSIAVGENVCITTSKDILDAIAHNEGVGRYQIALGYASWGKNQLEDEIARGDWLIC-DADMDLIFNLP---YDDRWDAAYKKIGVDRTWLASEI--GHA 5UEB Chain:A ((6-147)) -------------------HHHMKSKLTVVYYDLES----------------------------NIAEEILSGNIMPDGNFLIQEI-PLFAPNLALNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENYLSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS

General information: TITO was launched using: RESULT: Template: 5UEB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 598 -43925 -73.45 -322.98 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -73.45 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_5UEB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UEB-query.scw PDB file : Tito_Scwrl_5UEB.pdb: