Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MNKLKLKGRPRKGKFECFSTQAWF-----------YAVQMVSNIGTAYALEKVFFPTRFKTVKGKIIRPR---LFDRYKMGTITVGKKLV-------------QQVEAS-YTNTALWFNAVLWKILDERCDDFELNAWFRSLDQNLMQFFFKSTLTVDDSIKIQ--VKKINQKNFKKL---KNYELLD---VFTLVVLAIQMSIEKKDMDSLDK------------ALYGYHQIRRTLQEHPIFSKFYENLLSTLEL---HFIQQGEHTF---------------------------------GSPIALMLWEYD---PMMSVHPQMRMATISEGKSVQQTISDASYLVKAQVCCFE---KYYC-------NSDLKATIRLYYSIPLGLSLLPNVDSTVLIPFAKKFFPLARFPEIIAQDKENMIAMTMMFGDELDWESLESMVGIAVLQEIVYFNEDRFVETHTRFWQERLGLIK-------------------------------- 3ALX Chain:A ((8-549)) CSGPTTIRGQFSNMSLSLLDLYLGRGYNVSSIVTMTSQGMYGGTYLVEKPSMYRVFEVGVIRNPGLGAPVFHMTNYLEQPVSNDLSNCMVALGELKLAALCHGEDSITIPYQGSGKGVSFQLVKLGVWKSPTDMQSWVPLSTDDPVIDRLYLSSHRGVIADNQAKWAVPTTRT-DDKLRMETCFQQACKGKIQALCENPEWAPLKDNRIPSYGVLSVDLSLTVELKIKIASGFGPLITHGSGMDLYKSNHNNVYWLTIPPMKNLALGVINTLEWIPRFKVSPYRFTVPIKEAGGDCHAPTYLPAEVDGDVKLSSNLVILPGQDLQYVLATYDTSRVEHAVVYYVYSPSRSFSYFYPFRLPIKGVPIELQVECFTWDQKLWCRHFCVLADSESGGHITHSGMVGMGVSCCPKIVQQ---LGSDVLLPLTHERINTSMNKSIHIVVTMAKSLENSVE--NKIVSLDPSEA----GPPRYLKDRYRFYLEHLSLAIYESTKKDEGWYFMTLEKNISVQRFCLHLKLYE

General information: TITO was launched using: RESULT: Template: 3ALX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2029 -55674 -27.44 -150.88 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -27.44 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.62 QMean score : -0.008

(partial model without unconserved sides chains): PDB file : Tito_3ALX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ALX-query.scw PDB file : Tito_Scwrl_3ALX.pdb: