Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------MHPYAPTLQRVL-LFGLFFILIFLGFNILKYFIVPVLWAAIIAYMTWPI---YLRIQHFFGENRNNLNATVMISLVILIVGVPF-----------TCAIFILQHEGR--NLYFDLQRQV-----FS-GH---LSVPDFIRNLPFIGKEISRT--------LNEINTDPNSTIQNIAAWVQSHL-SYGRVLLNEI------SKNIVKLCFAILSLFFFYRDGHTILTQVSKALEMVIGPRIHH-------YLDTISETTRAVVYGVGLTAIAQAVLAGLSYFVAGVPNPMVLTIATFLLALIPFGTPVSYLGVGLWLFSQGQTMEAIGVILWGVLIVSSSDNVIRPLVISGATQIPFLLIMFGVLGGIASFGLVGLFIGPVILAVLLAIWREWLHETIDPEPMPKTTMIYDSDDDLPPSKN 4RP9 Chain:A ((6-456)) NIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGTIGQLIAVGILVAC-GSSILIIPGFIPMFFSNATIGVFANHFG--GWRAALKICLVMGMIEIFGCVWAVKLTG-------------------MSAWMGMADWSI----LAPPMMQGFFSIGIAFMAVIIV---IALAYMFFAGRALRAEEDA

General information: TITO was launched using: RESULT: Template: 4RP9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1457 -185339 -127.21 -540.35 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -127.21 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.227

(partial model without unconserved sides chains): PDB file : Tito_4RP9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RP9-query.scw PDB file : Tito_Scwrl_4RP9.pdb: