Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQFAVIGNPIEQSRSPELHHA----FAEKTGVDLNYQKRLAPLDGFESSMRS-FFAEGGSGMNVTVPFKEQAFALCNVLTERAQIAKAVNTLWMENGKLHGDNTDGQGLVAAIQALEWNLENTTILILGAGGATRGVIYPLVQAGAKKIVI--ANRTLARAEQLVDDLKTAVPQAQLQAISLNDLEGDFDIVINATSASLSGDALQLPEKLK-----FKYAYEMAYGKPSSFLDQAKQRNVPYAEGFGMLVGQAIEAFSIWNGVRPQLKDFL 3PHJ Chain:A ((4-246)) --KSFGVFGNPIKHSKSPLIHNACFLTFQKELRFLGHYHPILLPL---ESHIKSEFLHLGLSGANVTLPFKERAFQVCDKIKGIALECGAVNTLVLENDELVGYNTDALGFYLSLK-----YQNA--LILGAGGSAKALACELKKQGLQVSVLNRSSRGLDFFQRLGCDCFMEPPKSA------------FDLIINATS-----ELPLNKEVLKGYFKEGKLAYDLAYGFLTPFLSLAKELKTPFQDGKDMLIYQAALSFEKFS----------

General information: TITO was launched using: RESULT: Template: 3PHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1215 5856 4.82 26.26 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 4.82 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_3PHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PHJ-query.scw PDB file : Tito_Scwrl_3PHJ.pdb: