Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MTDITLKTPVEMRNVQLKVYDNTDN--SILIDTQVASLTSYDIKLPST--KLNRLYRIEVTTQGSSQVFDPTKSEYQNISGVYHAFITPSSISNKTQLISPSSEAIYQRAVI--RSGQLPNETIIPTRIEQLHVDLASQDVYRSLLNAFKDTNIPSLSPASTLTILNLLKYSTTKPSTYVDSYLCFGYLQYWSSIHNTNSPYQDLTQSLATDLKDGYLDGKKLI-GDKTSFTPIFTSAPDNIDPAKNTLLDIAANQKVTRDYFATNLRAATFKLADLQDQSHLDPIGYDLLDKKQYSGIIPAGNTSEFFRIDGAGDYRRAVGFSGITATCNG-SAYPCKQGLTSINISDPKLPATDYLVGHYEDSTTGCQLNFRANGQIELIKGSQSYRSALSADSTDKLLQTDAASYSYLLNSSSSEPNNSTQQYNFIQLHIKANKIVSAQAGLDSRKAPDVLQTTQLEYSFS 4FHL Chain:A ((2-392)) GSMTLSSNQYQLPLNVRPYTTTWCSQSPSCSNLLAIGHDTGITIYCASEESTGLTLQELFTIQTGLPTLHLSFSSSCSYSEN-SPVYSLFLACVCQDNTVRLIITKNETIITQHVLGGKSGH-----------HNFVNDIDIADVYSAD-NRLAEQVIASVGDDCTLIIWRLTDEGPILAGYPLSSP--GISVQFRPS------------------------NPNQLIVGERNGNIRIFDWTLNLTELVKNPWLLTLNTLPLVNSSLA-NVRWI----------------------GSDGSGILAMCKSGAWLRWNL---------FKNLLPNVQGISLFPSLLG------ACPHPRYMDYFATAHSQ-----------HGLIQLINTYE--------------------------KDSNSIPIQLGMPIVDFCWHQDGSHLAIATEG-------SVLLTRLMGFT--

General information: TITO was launched using: RESULT: Template: 4FHL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1932 32102 16.62 96.40 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 16.62 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.128

(partial model without unconserved sides chains): PDB file : Tito_4FHL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FHL-query.scw PDB file : Tito_Scwrl_4FHL.pdb: