Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALTSIAPVINQYGVTVSTYSEIVEHLKEKYREIYGQDVYLENDSQDGQWIGVIARVVADCNAAVADVYSSMSPSTAGTDALSRNVKINGIRRAVATKSTVSVVLVGAAGTIINNGIVSDKNNNRWLLPTQVVIPPEGEIFVAASAEKPGAILALPNSITTISTPTRGWQSVNNPQASTLGAPVESNVKLRQRQALSTAIPSRSYTEGILGALF-S-LDGVSRCKVYENKKSFVDPLELPPNSLSVVVAGGDD--------QLIAETIRVKKAPGCDLYGNTTVIRPTVYGDPVEIQYWRPVQKSIGLRFELTTNSDY----TVDIGEQIKSATADYINQ--LDIGDRIAINKLYVPAGLYGALDARSYEIESLQLTVDGVPVEGDYTLDFNAVAYCDSDDIEISVAGGG 3H2T Chain:B ((342-474)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATDYDTFVSERFGSIIQAVQTFTDSTK--------PGYAFIAAKPKSGLYLTTVQREDIKNYLKDYNLAPIT------------------PSIISPNYLFIKTNLKVTYALNKLQESEQWLEGQIIDKIDRYYTEDVEIFNSSFAKSKMLTYV--DDA-DH-S------------------------------------------

General information: TITO was launched using: RESULT: Template: 3H2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 313 7253 23.17 61.99 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.51 3D Compatibility (PKB) : 23.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_3H2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H2T-query.scw PDB file : Tito_Scwrl_3H2T.pdb: