Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRPDSKSMNYQLLKTFSRQPIQFGRFLARLLAGLVNTLKITRTSKSIELNLRIALPYLTPQQRIAITEKAVRNELTSYFEFLSIWGSSNSKNISRIHRIEGEHFFHEALAAKKGVVLIVPHFGTWEVMNAWCAQF-TSMTILYKPVKNADADRFVREARSREQANLVPTDE---SGVRQIFKALKQGETTVILPDHTPNVGGDMVNYFGVPLASSNLSAKLIQKTKAKALFLYAIRNENDGFTIHIEPMDEKIYEGTADDGTYVIHQAIEQLIYQYPEHYHWSYKRFKANPALDNIYNIDPTEALKIVDRLKAEALKTSTQPEPIQTSVM 5F2T Chain:B ((37-295)) --------------------------------AR-Y--AARNGGPEQLRRNLARVVGKPPADVPDDLIRASLASYARYWREAFRLPAMDHGRLGEQ-LDVIDIDHLWSALDAGRGAVLALPHSGNWDMAGVWLVQNYGPFTTVAERLKPESLYRRFVEYRESLGFEVLPLTGGERPPFEVLAERLTDNRPICLMAERDLTRSGVQVDFFGEATRMPAGPAKLAIETGAALFPVHCWFEGD-GWGMRVYPELDT-SSGDVTAITQALADRFAANIATYPADWHMLQPQWIADLSDERR----------------------------------

General information: TITO was launched using: RESULT: Template: 5F2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1274 -23243 -18.24 -91.15 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -18.24 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_5F2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F2T-query.scw PDB file : Tito_Scwrl_5F2T.pdb: