Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIPIALIIWLVRWIKGNTHRKNYY-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4ZMU Chain:A ((5-345)) MLACPLPPDEALRQQALD----DMALVDTPAEHYLDALVELARETF--GVKTVL-ISLIDHDRQWFKARIGLDAEQTPRDLSFCGHAILASEPLMVTDASRDPRFHDNPLVTGPPFIRFYAGEPLHASNGQAIGTLCLIDPSPRLLDLREGRQLNRLSILAEGYLQLRSLTEHTRFLRQEIDREQRKSLLDPLTQLWNRAGFHALHQHELELARASDQRIGIIYSDIDHFKRINDTLGHRAGDSVLREAASRLRAALRPEDLLARFGGEEFVAMVRVRETTELTMIANRIRELMEATPIDCAGTSVPVTISAGCTLAGSGEEPERALARADAALYDAKRAGRNRVVSV

General information: TITO was launched using: RESULT: Template: 4ZMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 89 -1701 -19.11 -29.32 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -19.11 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_4ZMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZMU-query.scw PDB file : Tito_Scwrl_4ZMU.pdb: