Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA 2KON Chain:A ((1-82)) --------------MNVAHYRGYEIEPGHQYRDDIRKYVPYA---LIRKVGVPDRTPIP--TTYPEFYDLEADAERVSIACAKIIIDSHLDRHDQGLADLG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2KON.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 328 -29834 -90.96 -363.82 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.45 3D Compatibility (PKB) : -90.96 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.45 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_2KON.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KON-query.scw PDB file : Tito_Scwrl_2KON.pdb: