Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MEWFCK------FLLFLEVIHVY----GFVFLTNKKAGSTMDHLYIKY----IHQ------FSVLVLGTLIIAGIVAFIIQG-RDILTVHQEFFSYAFGIPLIAIRSD-----NKKWKKRYYILLLFLCFY------LPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK------------------------------ 5K01 Chain:A ((1-214)) DTKEQRILRYVQQNAKPGDPQSVLEAIDTYCTQKEWAMNVGDAKGQIMDAVIREYSPSLVLELGAYCGYSAVRMARLLQPGARLLTMEINPDCAAITQQMLNFAGLQDKVTILNGASQDLIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEKCGLLRKGTVLLADNVIVPGTPDFLAYVRGSSSFECTHYSSYLEYMKVVDGLEKAIYQGPS

General information: TITO was launched using: RESULT: Template: 5K01.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 707 -76807 -108.64 -529.70 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -108.64 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_5K01.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K01-query.scw PDB file : Tito_Scwrl_5K01.pdb: