Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------MNGFMDK---LSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSIVVVINNLP------------FFSDATKG-TLSNLFGN-----------GQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGAVSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGWQIKMPDGVPPAVTKPFAALIPAVVTLTI------FLVINAVMTGIF------NANL---HDVVYEVIQKPLTGLGSSLPATLIALFFVQFLWFFGLHGQII-VNSVMDPIWNTLMLDNLEAYQHGKEL---PHIITKPFMETFTVGI--GGSGMTLAVVLLMAFVLKKKQY-----RDVGRLALAPGIFNVNEPAIFGLPIVLNATILIP-----------------WVVAPLIVTTFNYLVMAA-------GLVPAPTGVSVPWTVPIIASGVLAT----------NSWLGGMLQVID---------------FVIVAFIWYPFLKVLDKQPDLDIA 3JC6 Chain:E ((5-650)) ISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVPIFGYSELRRHYSQLDDN-INSLLLVGFGGVIDLEAFLEIDPQEQSFRRDIYVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELNDLSKRKQRKKQIHEYEGVLEEYYSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVKRLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLHKMFARMG-------IPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSISASEFVEALTALLEVGNSAQKLTNLRKRWVSNFWLSWDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYRNPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGTKKPILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL---

General information: TITO was launched using: RESULT: Template: 3JC6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1836 -105174 -57.28 -255.28 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain E : 0.63 3D Compatibility (PKB) : -57.28 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.029

(partial model without unconserved sides chains): PDB file : Tito_3JC6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JC6-query.scw PDB file : Tito_Scwrl_3JC6.pdb: