Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFHSTPNKGGTFDMTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQLLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFYTSEEKAKAHLDAGVKRVVISAPA-GAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPMAYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAIGLVIPELQGKLQGHAQRVPVVDGSLTELVSVL-KTKVTADQVNEAIKKHTVDNPSFGYDDRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFANL 3KSZ Chain:O ((2-334)) ---------------AVKVAINGFGRIGRLAFRRIQEV-EGLEVVAVNDLTDDDMLAHLLKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPW-KDLNIDVVLECTGFYTDKDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASSTTNSLAPVAKVLNDDFGLVEGLMTTINAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIGKVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNAS--NESFGYTEDEIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL

General information: TITO was launched using: RESULT: Template: 3KSZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 1983 -56735 -28.61 -171.40 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain O : 0.87 3D Compatibility (PKB) : -28.61 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_3KSZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KSZ-query.scw PDB file : Tito_Scwrl_3KSZ.pdb: