Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAKDEYFLETSSSKRIIVCLLDVFVSISTIRYLFFIARCENMFHNREVRKKLSNGGQKMKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTITYGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADTIIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYFPKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIKHFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAH-LGKQNGRILEVTKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITENEHQYLVKHLQNY--KKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFAKLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILRIKSKKKEAQTLPPIVENEEAE-VELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEAQEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKG-ITLCKAVANEPLLTSAEMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIKAMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSKGKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENEVE 1MW9 Chain:X ((83-588)) ------------------------------------------------------------------------------------------------------------------------------------------YEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWH-LREVIG-GDDARYSRVVFNEITKNAIRQAFN--KPGELNIDRVNAQQARRFMDRVVGYMVSPLLW-----KKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLP---------ESPNQYA-------REAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPAL----EDRILPAVNKGDALTLVELTPAQHFTKPPARFSEASLVKEL-----------------EKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENNLDQVANHEAEWKAVLD-------HFFSDFTQQLDKAEKDPEEGGMR-------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1MW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 2346 82773 35.28 169.27 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain X : 0.67 3D Compatibility (PKB) : 35.28 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1MW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MW9-query.scw PDB file : Tito_Scwrl_1MW9.pdb: