Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEAVEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDELPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAYRLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEAISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQENGVTKLVKKSN 4H0E Chain:B ((52-82)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELMQQFGVSRHTIRKAIGDLVSQGLLYSVQG---------

General information: TITO was launched using: RESULT: Template: 4H0E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 41 -9478 -231.17 -305.74 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -231.17 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.728

(partial model without unconserved sides chains): PDB file : Tito_4H0E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H0E-query.scw PDB file : Tito_Scwrl_4H0E.pdb: